A 25-year-old man has died following a three-vehicle collision on the A9 at Dunblane yesterday evening.
Police say this morning that the Honda Civic driver's 26-year-old female passenger was seriously injured in the crash, which happened at about 6.25pm.
The 41-year-old driver of a Mercedes Sprint van was also injured and remains in hospital.
The collision, north of the Keir roundabout, also involved a Volvo articulated lorry.
The A9 was closed in both directions and reopened at about midnight.
Sgt Michael Montague, of Forth Valley road policing unit appealed for anyone with information to contact police.